CD3 epsilon antibody (AA 23-207)

Catalog No. ABIN3043805

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Binding Specificity: AA 23-207

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: CD3e molecule, epsilon (CD3-TCR complex)
Protein Name: T-cell surface glycoprotein CD3 epsilon chain

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD3 epsilon is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD3 epsilon antibody (ABIN3043805)

Guo, Qian, Zhang: "The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis." in: BMC ophthalmology, Vol. 16, pp. 66, (2017) (PubMed).

Chen, Liu, Xu, Yang, Zhang, Zhang: "Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 1063-1067, (2015) (PubMed).

Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

Li, Geng, Yin, Chen, Yang, Wu, Li, Du, Schmidt, Przybylski: "Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients." in: Journal of translational medicine, Vol. 8, pp. 47, (2010) (PubMed).

Xie, Jia, He, Gao: "Morphological observation of tumor infiltrating immunocytes in human rectal cancer." in: World journal of gastroenterology, Vol. 12, Issue 11, pp. 1757-60, (2006) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E (CD3E Products)

Synonyms: cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody

Background: CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Synonyms: CD3 epsilon antibody|CD3e antibody|CD3E antibody|CD3e antigen epsilon polypeptide (TiT3 complex) antibody|CD3E antigen epsilon polypeptide antibody|CD3E antigen, epsilon subunit antibody|CD3e molecule epsilon antibody|CD3e molecule, epsilon (CD3 TCR complex) antibody|CD3e molecule, epsilon (CD3-TCR complex) antibody|CD3E_HUMAN antibody|IMD18 antibody|T cell antigen receptor complex epsilon subunit of T3 antibody|T cell surface antigen T3/Leu 4 epsilon chain antibody|T cell surface glycoprotein CD3 epsilon chain antibody|T-cell surface antigen T3/Leu-4 epsilon chain antibody|T-cell surface glycoprotein CD3 epsilon chain antibody|T3E antibody|TCRE antibody

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway