FCER2 antibody (AA 50-331)

Catalog No. ABIN3043829

Product Details anti-FCER2 Antibody

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Binding Specificity: AA 50-331

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FCER2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Low affinity immunoglobulin epsilon Fc receptor(FCER2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Low affinity immunoglobulin epsilon Fc receptor(FCER2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
Protein Name: Low affinity immunoglobulin epsilon Fc receptor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for CD23 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for FCER2

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Alternative Name: FCER2 (FCER2 Products)

Synonyms: BLAST-2 antibody, CD23 antibody, CD23A antibody, CLEC4J antibody, FCE2 antibody, IGEBF antibody, Fcer2a antibody, Fc fragment of IgE receptor II antibody, FCER2 antibody, Fcer2 antibody

Background: CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Synonyms: Blast 2 antibody|BLAST-2 antibody|Blast2 antibody|C type lectin domain family 4 member J antibody|C-type lectin domain family 4 member J antibody|C-type lectin domain family 4, member J antibody|CD 23 antibody|CD 23A antibody|CD23 antibody|CD23 antigen antibody|CD23A antibody|CLEC 4J antibody|CLEC4J antibody|Fc epsilon RII antibody|Fc fragment of IgE low affinity II receptor for antibody|Fc fragment of IgE, low affinity II, receptor for (CD23) antibody|Fc of IgE, low affinity II, receptor for (CD23) antibody|Fc receptor IgE low affinity II alpha polypeptide antibody|Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody|Fc-epsilon-RII antibody|FCE 2 antibody|FCE2 antibody|FCER 2 antibody|Fcer2 antibody|FCER2_HUMAN antibody|FCER2A antibody|FceRII antibody|IgE binding factor antibody|IgE receptor lymphocyte antibody|IgE-binding factor antibody|IGEBF antibody|Immunoglobulin E binding factor antibody|Immunoglobulin E receptor, low affinity II antibody|Immunoglobulin E-binding factor antibody|LEUKOCYTE ANTIGEN CD23 antibody|Low Affinity IgE Receptor antibody|Low affinity immunoglobulin epsilon Fc receptor antibody|Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody|Low affinity immunoglobulin epsilon Fc receptor soluble form antibody|Ly-42 antibody|Ly42 antibody|Lymphocyte antigen CD23 antibody|Lymphocyte IgE receptor antibody|MGC93219 antibody

Gene ID: 14128

UniProt: P20693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process