HSP90AA1 antibody (AA 2-365)
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- Target See all HSP90AA1 Antibodies
- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
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Binding Specificity
- AA 2-365
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HSP90AA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Purpose
- Rabbit IgG polyclonal antibody for Heat shock protein HSP 90-alpha(HSP90AA1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Heat shock protein HSP 90-alpha(HSP90AA1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: heat shock protein 90 kDa alpha (cytosolic), class A member 1
Protein Name: Heat shock protein HSP 90-alpha - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Hsp90 recombinant protein (Position: P2-V365). Human Hsp90 shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90.
- Isotype
- IgG
- Top Product
- Discover our top product HSP90AA1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for Hsp90 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).
: "Heat shock protein 90-mediated inactivation of nuclear factor-?B switches autophagy to apoptosis through becn1 transcriptional inhibition in selenite-induced NB4 cells." in: Molecular biology of the cell, Vol. 22, Issue 8, pp. 1167-80, (2011) (PubMed).
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The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).
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- Target
- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
- Alternative Name
- HSP90AA1 (HSP90AA1 Products)
- Background
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Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene. The gene, HSP90AA1, encodes the human stress-inducible 90- kDa heat shock protein alpha (Hsp90A). Complemented by the constitutively expressed paralog Hsp90B which shares over 85 % amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.
Synonyms: D6S182 antibody|FLJ26984 antibody|FLJ31884 antibody|Heat shock 86 kDa antibody|heat shock 90 kDa protein 1 alpha antibody|Heat shock protein 90 kDa alpha cytosolic class A member 1 antibody|Heat shock protein 90 kDa alpha cytosolic class B member 1 antibody|Heat shock protein HSP 90 alpha antibody|Heat shock protein HSP 90 beta antibody|Heat shock protein HSP 90-alpha antibody|HS90A_HUMAN antibody|HSP 84 antibody|HSP 86 antibody|Hsp 90 antibody|HSP84 antibody|HSP86 antibody|Hsp89 antibody|Hsp90 antibody|HSP90 Beta antibody|HSP90A antibody|HSP90AA1 antibody|HSP90AB1 antibody|HSP90B antibody|HSP90N antibody|HSPC1 antibody|HSPC2 antibody|HSPCA antibody|HSPCAL1 antibody|HSPCAL4 antibody|HSPCB antibody|HSPN antibody|LAP2 antibody|Lipopolysaccharide associated protein2 antibody|LPS associated protein 2 antibody|NY REN 38 antigen antibody|Renal carcinoma antigen NY-REN-38 antibody - Gene ID
- 3320
- UniProt
- P07900
- Pathways
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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