RALB antibody (C-Term)

Catalog No. ABIN3043919

Product Details anti-RALB Antibody

Target: RALB (Ralb) (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein) (Ralb))

Binding Specificity: AA 166-203, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RALB antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Ras-related protein Ral-B(RALB) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DKVFFDLMRE IRTKKMSENK DKNGKKSSKN KKSFKERC

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Ras-related protein Ral-B(RALB) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: v-ral simian leukemia viral oncogene homolog B
Protein Name: Ras-related protein Ral-B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RALB (166-203aa DKVFFDLMREIRTKKMSENKDKNGKKSSKNKKSFKERC), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RALB

Target: RALB (Ralb) (V-Ral Simian Leukemia Viral Oncogene Homolog B (Ras Related, GTP Binding Protein) (Ralb))

Alternative Name: RALB (Ralb Products)

Synonyms: dRalb antibody, 5730472O18Rik antibody, Ral B antibody, ralb antibody, xralb antibody, zgc:100801 antibody, KRAS2 antibody, k-ras antibody, RAS like proto-oncogene B antibody, v-ral simian leukemia viral oncogene B antibody, v-ral simian leukemia viral oncogene homolog B L homeolog antibody, v-ral simian leukemia viral oncogene homolog Ba (ras related) antibody, v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) antibody, RALB antibody, Ralb antibody, ralb.L antibody, ralba antibody, ralb antibody

Background: Ras-related protein Ral-B (RalB) is a protein that in humans is encoded by the RALB gene on chromosome 2. This protein is one of two isoforms of the Ral protein, the other being RalA, and part of the Ras GTPase family. As a Ras GTPase, RalB functions as a molecular switch that becomes active when bound to GTP and inactive when bound to GDP. RalB can be activated by RalGEFs and, in turn, activate effectors in signal transduction pathways leading to biological outcomes. Additionally, Ral proteins have been associated with the progression of several cancers, including bladder cancer and prostate cancer. While the above functions appear to be shared between the two Ral isoforms, their differential subcellular localizations result in their differing involvement in certain biological processes. In particular, RalB is more involved in apoptosis and cell motility. Moreover, RalB specifically interacts with Exo84 to assemble the beclin-1-VPS34 autophagy initiation complex, and with Sec5 to activate the innate immune response via the Tank-binding kinase 1 (TBK1).

Synonyms: 5730472O18Rik antibody|dRalb antibody|GTP binding protein antibody|Ralb antibody|RALB_HUMAN antibody|RAS like protein B antibody|Ras related GTP binding protein B antibody|Ras-related protein Ral-B antibody|v ral simian leukemia viral oncogene homolog B (ras related GTP binding protein) antibody|v ral simian leukemia viral oncogene homolog B (ras related) antibody|V ral simian leukemia viral oncogene homolog B antibody

Gene ID: 5899

UniProt: P11234

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events