BAG3 antibody (C-Term)

Catalog No. ABIN3043976

Product Details anti-BAG3 Antibody

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Binding Specificity: AA 491-506, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 3(BAG3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: LEKLEQKAID VPGQVQ

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 3(BAG3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: BCL2-associated athanogene 3
Protein Name: BAG family molecular chaperone regulator 3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Bag3(491-506aa LEKLEQKAIDVPGQVQ), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for BAG3 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BAG3

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Alternative Name: BAG3 (BAG3 Products)

Synonyms: bag3-A antibody, MGC53113 antibody, zgc:100859 antibody, BAG3 antibody, ATBAG3 antibody, BCL-2-associated athanogene 3 antibody, T28J14.160 antibody, T28J14_160 antibody, BAG-3 antibody, BIS antibody, CAIR-1 antibody, MFM6 antibody, AA407278 antibody, Bis antibody, mg638 antibody, BCL2 associated athanogene 3 L homeolog antibody, BCL2-associated athanogene 3 antibody, BCL2 associated athanogene 3 antibody, BCL-2-associated athanogene 3 antibody, Bcl2-associated athanogene 3 antibody, bag3.L antibody, bag3 antibody, BAG3 antibody, Bag3 antibody

Background: BAG family molecular chaperone regulator 3(BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones. BAG3 mutations are responsible for familial dilated cardiomyopathy. BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus. In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes. This process is called chaperone-assisted selective autophagy(CASA) and is essential for maintaining muscle activity in flies, mice and men.

Synonyms: BAG 3 antibody|BAG family molecular chaperone regulator 3 antibody|BAG-3 antibody|Bag3 antibody|BAG3_HUMAN antibody|Bcl 2 binding protein antibody|Bcl-2-associated athanogene 3 antibody|Bcl-2-binding protein Bis antibody|BCL2 associated athanogene 3 antibody|BCL2 binding athanogene 3 antibody|BIS antibody|Docking protein CAIR 1 antibody|Docking protein CAIR-1 antibody

UniProt: O95817