SLC2A5 antibody (Middle Region)

Catalog No. ABIN3044131

Product Details anti-SLC2A5 Antibody

Target: SLC2A5 (Solute Carrier Family 2 (Facilitated Glucose/fructose Transporter), Member 5 (SLC2A5))

Binding Specificity: AA 232-251, Middle Region

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC2A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 5(SLC2A5) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.

Sequence: ALQTLRGWKD VHLEMEEIRK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 5(SLC2A5) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.
Gene Name: solute carrier family 2(facilitated glucose/fructose transporter), member 5
Protein Name: Solute carrier family 2, facilitated glucose transporter member 5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse SLC2A5(232-251aa ALQTLRGWKDVHLEMEEIRK), different from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-SLC2A5 antibody (ABIN3044131)

Li, Byrd, Doh, Dixon, Lee, Tiwari, Ecelbarger: "Absence of renal enlargement in fructose-fed proximal-tubule-select insulin receptor (IR), insulin-like-growth factor receptor (IGF1R) double knockout mice." in: Physiological reports, Vol. 4, Issue 23, (2018) (PubMed).

Target Details for SLC2A5

Target: SLC2A5 (Solute Carrier Family 2 (Facilitated Glucose/fructose Transporter), Member 5 (SLC2A5))

Alternative Name: SLC2A5 (SLC2A5 Products)

Synonyms: GLUT-5 antibody, GLUT5 antibody, AI526984 antibody, Glut5 antibody, Slc5a antibody, glut5 antibody, slc2a7 antibody, fb58f04 antibody, wu:fb58f04 antibody, solute carrier family 2 member 5 antibody, solute carrier family 2 (facilitated glucose/fructose transporter), member 5 antibody, solute carrier family 2 (facilitated glucose transporter), member 5 antibody, solute carrier family 2, facilitated glucose transporter member 5 antibody, SLC2A5 antibody, Slc2a5 antibody, LOC489640 antibody, slc2a5 antibody

Background: GLUT5(Glucose transporter 5), also known as SLC2A5, is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.

Synonyms: Facilitated glucose transporter member 5 antibody|Fructose transporter antibody|glucose transporter like protein 5 antibody|Glucose transporter type 5 antibody|Glucose transporter type 5 small intestine antibody|GLUT 5 antibody|GLUT-5 antibody|GLUT5 antibody|GTR5_HUMAN antibody|SLC 2A5 antibody|SLC2A5 antibody|small intestine antibody|Solute carrier family 2 antibody