HSPH1 antibody (C-Term)

Catalog No. ABIN3044218

Product Details anti-HSPH1 Antibody

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Binding Specificity: AA 713-733, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Heat shock protein 105 kDa(HSPH1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequence: EVMEWMNNVM NAQAKKSLDQ D

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Heat shock protein 105 kDa(HSPH1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: heat shock 105 kDa/110 kDa protein 1
Protein Name: Heat shock protein 105 kDa

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp105(713-733aa EVMEWMNNVMNAQAKKSLDQD), different from the related mouse sequence by one amino acid, and different from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSPH1

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Alternative Name: HSPH1 (HSPH1 Products)

Synonyms: hsp105 antibody, hsp105a antibody, hsp105b antibody, hsp110 antibody, hsph1b antibody, Hsp105 antibody, HSP105 antibody, HSP105A antibody, HSP105B antibody, NY-CO-25 antibody, 105kDa antibody, AI790491 antibody, Hsp110 antibody, hsp-E7I antibody, hsph1 antibody, hsph1a antibody, heat shock protein family H (Hsp110) member 1 antibody, heat shock protein family H (Hsp110) member 1 S homeolog antibody, heat shock protein 105 kDa antibody, heat shock 105kDa/110kDa protein 1 antibody, heat shock protein family H (Hsp110) member 1 L homeolog antibody, hsph1 antibody, hsph1.S antibody, HSPH1 antibody, LOC100546590 antibody, Hsph1 antibody, hsph1.L antibody

Background: HSP105(HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.

Synonyms: Antigen NY CO 25 antibody|Antigen NY-CO-25 antibody|DKFZp686M05240 antibody|Heat shock 105kD alpha antibody|Heat shock 105kD antibody|Heat shock 105kD beta antibody|Heat shock 105 kDa protein 1 antibody|Heat shock 105 kDa protein antibody|Heat shock 105 kDa/110 kDa protein 1 antibody|Heat shock 110 kDa protein antibody|Heat shock 110 kDa protein antibody|Heat shock protein 105 kDa antibody|HS105_HUMAN antibody|HSP105 antibody|HSP105A antibody|HSP105B antibody|HSP110 antibody|HSPH 1 antibody|HSPH1 antibody|KIAA0201 antibody|NY CO 25 antibody

UniProt: Q92598