ALPL antibody (Alkaline Phosphatase, Liver/bone/kidney) (AA 21-35)

Details for Product anti-ALPL Antibody No. ABIN3044314, Supplier: Log in to see
Antigen
  • ALP
  • iap
  • alpl
  • AP-TNAP
  • APTNAP
  • HOPS
  • TNAP
  • TNSALP
  • Akp-2
  • Akp2
  • PHOA
  • TNS-AP
  • alkaline phosphatase, liver/bone/kidney L homeolog
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, intestinal, gene 2
  • alkaline phosphatase, liver/bone/kidney pseudogene
  • alpl.L
  • ALPL
  • alpi.2
  • LOC100031702
  • Alpl
Epitope
AA 21-35, N-Term
20
15
9
8
8
7
6
6
6
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
203
75
54
20
19
2
2
2
1
1
1
Host
Rabbit
131
89
4
3
1
Clonality
Polyclonal
Conjugate
This ALPL antibody is un-conjugated
13
11
11
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
142
105
58
49
48
24
23
21
13
10
6
3
2
1
Options
Supplier
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Purpose Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase(21-35aa,EKEKDPKYWRDQAQE), different from the related rat and mouse sequences by two amino acids.
Sequence EKEKDPKYWR DQAQE
Isotype IgG
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: alkaline phosphatase, liver/bone/kidney
Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme(AP-TNAP/TNSALP)
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others ALPL products on genomics-online (e.g. as negative or positive controls)
Antigen Alkaline Phosphatase, Liver/bone/kidney (ALPL)
Alternative Name ALPL (ALPL Antibody Abstract)
Background Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Synonyms: Akp2 antibody|Alkaline phosphatase intestinal antibody|Alkaline phosphatase intestinal precursor antibody|Alkaline phosphatase liver/bone/kidney antibody|Alkaline phosphatase placental antibody|Alkaline phosphatase placental like 2 antibody|Alkaline phosphatase placental like antibody|Alkaline phosphatase placental type antibody|Alkaline phosphomonoesterase antibody|ALP 1 antibody|ALP antibody|ALP I antibody|ALP L antibody|ALP P antibody|ALPG antibody|ALPI antibody|ALPL antibody|ALPP antibody|ALPPL antibody|ALPPL2 antibody|AP TNAP antibody|FLJ40094 antibody|GCAP antibody|Germ cell alkaline phosphatase antibody|Glycerophosphatase antibody|HOPS antibody|IAP antibody|Intestinal alkaline phosphatase antibody|Kasahara isozyme antibody|Nagao isozyme antibody|OTTHUMP00000164357 antibody|Placental alkaline phosphatase antibody|Placental like alkaline phosphatase antibody|PLAP 1 antibody|PLAP antibody|PLAP like antibody|Regan isozyme antibody|Testicular and thymus alkaline phosphatase antibody|Tissue non specific alkaline phosphatase antibody|Tissue nonspecific ALP antibody|TNAP antibody|TNSALP antibody
UniProt P05186
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody (ABIN3044314) Anti-Alkaline Phosphatase antibody, IHC(P) IHC(P): Rat Small Intestine Tissue
Western Blotting (WB) image for anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody (ABIN3044314) All lanes: Anti ALPL () at 0.5ug/ml Lane 1: Human Placenta Tissue Lysate at 50ug Lane...
Western Blotting (WB) image for anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody (ABIN3044314) All lanes: Anti ALPL () at 0.5ug/ml Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug ...
Western Blotting (WB) image for anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody (ABIN3044314) anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibody (Image 4)
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