CDC42 antibody (Middle Region)

Catalog No. ABIN3044385

Product Details anti-CDC42 Antibody

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Binding Specificity: AA 121-138, Middle Region

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Cell division control protein 42 homolog(CDC42) detection. Tested with WB, IHC-P in Human,Bovin,Mouse,Rat.

Sequence: DDPSTIEKLA KNKQKPIT

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cell division control protein 42 homolog(CDC42) detection. Tested with WB, IHC-P in Human,Bovin,Mouse,Rat.
Gene Name: cell division cycle 42(GTP binding protein, 25 kDa)
Protein Name: Cell division control protein 42 homolog(Cdc42 homolog)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Bovine, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-CDC42 antibody (ABIN3044385)

Han, Zhuang, Chen, Zhu, Cai, Lu et al.: "Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and ..." in: PLoS ONE, Vol. 12, Issue 9, pp. e0185102, (2017) (PubMed).

Target Details for CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42 (CDC42 Products)

Synonyms: CDC42Hs antibody, G25K antibody, AI747189 antibody, AU018915 antibody, cdc42a antibody, wu:fb07d08 antibody, wu:fj62g06 antibody, zgc:63918 antibody, Xcdc42 antibody, cdc42hs antibody, g25k antibody, CDC42 antibody, CG12530 antibody, Cdc42Dm antibody, D-CDC42 antibody, D-Cdc42 antibody, D-cdc42 antibody, DCDC42 antibody, DCdc42 antibody, Dcdc42 antibody, Dm Cdc42 antibody, DmCDC42 antibody, Dmcdc42 antibody, Dmel\\CG12530 antibody, cdc42 antibody, cell division cycle 42 antibody, Cell division control protein 42 homolog antibody, cell division control protein 42 homolog antibody, cell division cycle 42 L homeolog antibody, CG12530 gene product from transcript CG12530-RC antibody, Rho family GTPase antibody, CDC42 antibody, Cdc42 antibody, cdc42 antibody, LOC100636735 antibody, LOC100637699 antibody, LOC100638834 antibody, cdc42.L antibody

Background: Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein(N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.

Synonyms: CDC 42 antibody|CDC42 antibody|CDC42_HUMAN antibody|CDC42Hs antibody|Cell division control protein 42 homolog antibody|Cell division cycle 42(GTP binding protein 25 kDa) antibody|Cell division cycle 42 antibody|Cell division cycle 42 isoform 1 antibody|Cell division cycle 42 isoform 2 antibody|dJ224A6.1.1(cell division cycle 42(GTP-binding protein, 25kD)) antibody|dJ224A6.1.1 antibody|dJ224A6.1.2(cell division cycle 42(GTP-binding protein, 25kD)) antibody|dJ224A6.1.2 antibody|G25K antibody|G25K GTP binding protein antibody|G25K GTP-binding protein antibody|Growth regulating protein antibody|GTP binding protein 25kD antibody|GTP binding protein 25 kDa antibody|Small GTP binding protein CDC42 antibody

UniProt: P60953

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling