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PPARA antibody (Peroxisome Proliferator-Activated Receptor alpha) (AA 393-412) Primary Antibody

PPARA Reactivity: Human, Mouse, Rat ICC, IHC (fro), IHC (p), WB Host: Rabbit Polyclonal
Pubmed (6) Independent Validation (1)
Catalog No. ABIN3044397
$240.00
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100 μg
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  • Target
    Binding Specificity
    AA 393-412, C-Term
    Reactivity
    Human, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This PPARA antibody is un-conjugated
    Application
    Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Sequence
    NIGYIEKLQE GIVHVLKLHL
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Gene Name: peroxisome proliferator-activated receptor alpha
    Protein Name: Peroxisome proliferator-activated receptor alpha(PPAR-alpha)
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Restrictions
    For Research Use only
  • Validation #300032 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Human Protein Atlas
    No.
    #300032
    Date
    12/13/2016
    Antigen
    PPARA
    Lot Number
    Method validated
    Immunohistochemistry
    Positive Control
    liver
    Negative Control
    Notes
    Passed. ABIN3044397 staining is consistent with RNA-seq data and published literature.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3044397
    Secondary Antibody
    HRP polymer (ThermoFisher Scientific, TL-125-PH)
    Full Protocol
    • Sample preparation:
      • Fixation and TMA preparation according to PMID: 22688270.
      • Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
      • Dry sections ON at RT and then back them for 12-24h at 50°C.
    • Deparaffinization and rehydration in xylene and graded alcohol series:
      • xylene twice for 3min.
      • 100% ethanol for 1min.
      • 95% ethanol for 1min.
      • 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
      • 70% ethanol for 1min.
      • 50% ethanol for 1min.
      • distilled H2O for 2min.
    • Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
      • Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
      • Allow slides to cool down to 90°C in the decloaking chamber.
    • Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
      • Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
      • Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
      • Rinse sections 2x in wash buffer.
      • Incubate sections with primary rabbit anti-PPARA antibody (antibodies-online, ABIN3044397) diluted 1:1100 for 30min.
      • Rinse sections 3x in wash buffer.
      • Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
      • Rinse sections 2x in wash buffer.
      • Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
      • Rinse sections in distilled H2O.
    • Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
      • Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
      • Rinse sections in tap water for 5min.
      • Rinse sections in lithium carbonate water diluted 1:5 for 5min.
      • Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
      • Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
    • Microscopy:
      • Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
    Experimental Notes
    Nuclear staining of PPARA with ABIN3044397 is observed in all tissues, consistent with RNA-seq data and supported by published work. ABIN3044397 does show also show some cytoplasmic and membrane staining but significantly weaker than nuclear staining.
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Yang, Fu, Hu, Luo, Hu, Wang: "PIK3R3 regulates PPARα expression to stimulate fatty acid β-oxidation and decrease hepatosteatosis." in: Experimental & molecular medicine, Vol. 50, Issue 1, pp. e431, 2018 (PubMed).

    Cheng, Ge, Lan, Ma, Chi, Kuang, Sun, Zhao, Liu, Feng, Huang, Luo, Li, Zhang, Hu, Xu, Liu, Huo, Deng, Yang, Xi, Zhang, Siegenthaler, Chen: "Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity." in: Archives of toxicology, Vol. 92, Issue 1, pp. 469-485, 2017 (PubMed).

    Zhou, Zhang, Xu, Wang: "Curcumin ameliorates renal fibrosis by inhibiting local fibroblast proliferation and extracellular matrix deposition." in: Journal of pharmacological sciences, Vol. 126, Issue 4, pp. 344-50, 2015 (PubMed).

    Li, Chen, Peng, Zhou, Fang: "Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats." in: Bioelectromagnetics, Vol. 35, Issue 3, pp. 170-80, 2014 (PubMed).

    Shi, Zhang, Zhang, Zhang, Wu, Cheng, Li: "Chronic exposure to contaminated drinking water stimulates PPAR expression in mice livers." in: Chemosphere, Vol. 88, Issue 4, pp. 407-12, 2012 (PubMed).

    Xie, Wang, Zhang, Li, Can, Qing, Kung, Zhang: "Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice." in: Experimental and therapeutic medicine, Vol. 2, Issue 2, pp. 309-315, 2012 (PubMed).

  • Target
    Alternative Name
    PPARA (PPARA Antibody Abstract)
    Synonyms
    NR1C1, PPAR, PPARalpha, hPPAR, LOC100136415, PPARALPHA, nr1c1, xPPARalpha, xppara, 4933429D07Rik, AW742785, Nr1c1, PPAR-alpha, Ppar, peroxisome proliferator activated receptor alpha, peroxisome proliferator-activated receptor alpha, peroxisome proliferator activated receptor alpha L homeolog, PPARA, LOC100136415, ppara.L, Ppara, ppara
    Background
    Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85 % and 91 % DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

    Synonyms: hPPAR antibody|MGC2237 antibody|MGC2452 antibody|NR1C1 antibody|Nuclear receptor subfamily 1 group C member 1 antibody|OTTHUMP00000197740 antibody|OTTHUMP00000197741 antibody|Peroxisome Proliferator Activated Receptor alpha antibody|Peroxisome proliferator-activated receptor alpha antibody|PPAR antibody|PPAR-alpha antibody|PPARA antibody|PPARA_HUMAN antibody|PPARalpha antibody
    UniProt
    P23204
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
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