PPID antibody (C-Term)

Catalog No. ABIN3044408

Product Details anti-PPID Antibody

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Binding Specificity: AA 351-370, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Peptidyl-prolyl cis-trans isomerase D(PPID) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KQKIKAQKDK EKAVYAKMFA

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Peptidyl-prolyl cis-trans isomerase D(PPID) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: peptidylprolyl isomerase D
Protein Name: Peptidyl-prolyl cis-trans isomerase D(PPIase D)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PPID(351-370aa KQKIKAQKDKEKAVYAKMFA), different from the related mouse and rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPID

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: PPID (PPID Products)

Synonyms: PPID antibody, CYP-40 antibody, CYPD antibody, Cyp-40 antibody, CypD antibody, wu:fb18b07 antibody, zgc:86711 antibody, 4930564J03Rik antibody, Ppidl antibody, Ppif antibody, cyp-40 antibody, cypd antibody, peptidylprolyl isomerase D antibody, peptidylprolyl isomerase D (cyclophilin D) antibody, peptidylprolyl isomerase D (cyclophilin D) S homeolog antibody, PPID antibody, Ppid antibody, ppid antibody, ppid.S antibody

Background: Cyclophilin D, Peptidylprolyl isomerase D, also known as PPID, is an enzyme which in humans is encoded by the PPID gene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase(PPIase) family. The Cyclophilin D(PPID) gene contains 10 exons and spans 14.2 kb of genomic DNA. By fluorescence in situ hybridization, the PPID gene is mapped to chromosome 4q31.3. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant ciclosporin.

Synonyms: 40 kDa peptidyl-prolyl cis-trans isomerase antibody|40 kDa peptidyl-prolyl cis-trans isomerase D antibody|Cyclophilin related protein antibody|Cyclophilin-40 antibody|Cyclophilin-related protein antibody|CyclophilinD antibody|CYP-40 antibody|CYP40 antibody|CYPD antibody|Estrogen receptor-binding cyclophilin antibody|Peptidyl-prolyl cis-trans isomerase D antibody|Peptidylprolyl isomerase D antibody|PPIase D antibody|PPID antibody|PPID_HUMAN antibody|Rotamase antibody|Rotamase D antibody

UniProt: Q08752

Pathways: Nuclear Hormone Receptor Binding