HDAC8 antibody (N-Term)

Catalog No. ABIN3044454

Product Details anti-HDAC8 Antibody

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Binding Specificity: AA 18-33, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Histone deacetylase 8(HDAC8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: YIYSPEYVSM CDSLAK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Histone deacetylase 8(HDAC8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: histone deacetylase 8
Protein Name: Histone deacetylase 8(HD8)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC8(18-33aa YIYSPEYVSMCDSLAK), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HDAC8

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Alternative Name: HDAC8 (HDAC8 Products)

Synonyms: HD8 antibody, CDLS5 antibody, HDACL1 antibody, MRXS6 antibody, RPD3 antibody, WTS antibody, 2610007D20Rik antibody, RGD1562895 antibody, wu:fd19a02 antibody, zgc:66196 antibody, ATHDA8 antibody, HDA8 antibody, HISTONE DEACETYLASE 8 antibody, T27G7.14 antibody, T27G7_14 antibody, histone deacetylase 8 antibody, histone deacetylase 8 antibody, histone deacetylase 8 L homeolog antibody, CC1G_05302 antibody, hdac8 antibody, HDAC8 antibody, Hdac8 antibody, hdac8.L antibody, HDA08 antibody

Background: HDAC8, Histone deacetylase 8, is an enzyme that in humans is encoded by the HDAC8 gene, and is biologically involved in skull morphogenesis and metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex. Sequence analysis predicted that the 377-amino acid HDAC8 protein contains the 9 conserved HDAC blocks that are presumably important for catalytic function. HDAC8 shares 54 % sequence similarity with HDAC1 and HDAC2 and 39 % similarity with HDAC3, making it a class I HDAC. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Synonyms: CDA07 antibody|HD 8 antibody|HD8 antibody|HDAC 8 antibody|Hdac8 antibody|HDAC8_HUMAN antibody|HDACL 1 antibody|HDACL1 antibody|Histone deacetylase 8 antibody|Histone deacetylase like 1 antibody|RPD 3 antibody|RPD3 antibody

Pathways: Cellular Glucan Metabolic Process