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PIAS1 antibody (C-Term)

PIAS1 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3044474
  • Target See all PIAS1 Antibodies
    PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))
    Binding Specificity
    • 11
    • 10
    • 8
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 636-651, C-Term
    Reactivity
    • 49
    • 11
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 46
    • 4
    Rabbit
    Clonality
    • 46
    • 4
    Polyclonal
    Conjugate
    • 28
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PIAS1 antibody is un-conjugated
    Application
    • 41
    • 25
    • 24
    • 9
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Purpose
    Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
    Sequence
    DTASIFGIIP DIISLD
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
    Gene Name: protein inhibitor of activated STAT, 1
    Protein Name: E3 SUMO-protein ligase PIAS1
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1(636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Scortegagna, Berthon, Settas, Giannakou, Garcia, Li, James, Liddington, Vilches-Moure, Stratakis, Ronai: "The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion." in: JCI insight, Vol. 2, Issue 23, (2019) (PubMed).

  • Target
    PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))
    Alternative Name
    PIAS1 (PIAS1 Products)
    Synonyms
    2900068C24Rik antibody, Ddxbp1 antibody, GBP antibody, DDXBP1 antibody, GU/RH-II antibody, ZMIZ3 antibody, ddxbp1 antibody, gbp antibody, pias antibody, pias1-A antibody, xPAIS1 antibody, xPIAS1 antibody, zmiz3 antibody, protein inhibitor of activated STAT 1 antibody, protein inhibitor of activated STAT, 1 antibody, protein inhibitor of activated STAT, 1a antibody, protein inhibitor of activated STAT 1 L homeolog antibody, PIAS1 antibody, Pias1 antibody, pias1a antibody, pias1.L antibody
    Background
    E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

    Synonyms: DDXBP1 antibody|DEAD/H box binding protein 1 antibody|DEAD/H box-binding protein 1 antibody|E3 SUMO-protein ligase PIAS1 antibody|GBP antibody|Gu binding protein antibody|Gu-binding protein antibody|Pias1 antibody|PIAS1_HUMAN antibody|Protein inhibitor of activated STAT protein 1 antibody|RNA helicase II binding protein antibody|RNA helicase II-binding protein antibody
    UniProt
    O75925
    Pathways
    JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
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