beta Actin antibody (Actin, beta) (Cytosolic)

Details for Product anti-ACTB Antibody No. ABIN3044484, Supplier: Log in to see
Antigen
  • BRWS1
  • PS1TP5BP1
  • ACTA1
  • Actx
  • E430023M04Rik
  • beta-actin
  • ps1tp5bp1
  • act-2
  • actb
  • B-actin
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • ACT-5
  • ACTB
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • Beta-actin
  • actin beta
  • actin, beta
  • cytoplasmic actin OlCA1
  • beta-actin
  • Actin 5C
  • actin gamma 1
  • actin, beta 1
  • actin, beta L homeolog
  • beta actin
  • actin, cytoplasmic 1
  • ACTB
  • Actb
  • actb
  • LOC100174883
  • LOC100304816
  • Act5C
  • ACTG1
  • actb1
  • actb.L
  • LOC100136352
  • LOC109073280
Alternatives
anti-Cow (Bovine) beta Actin antibody for Immunocytochemistry
Epitope
Cytosolic, N-Term
160
42
17
17
10
8
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
607
356
309
109
87
85
69
59
55
45
42
42
34
32
28
23
16
16
16
15
10
9
9
8
7
6
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
377
256
14
7
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
36
31
19
6
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
595
252
183
141
114
101
59
51
37
17
14
13
12
9
8
2
2
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human,mouse,rat. No cross reactivity with other proteins.
Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Sequence Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys
Clone AC-15
Isotype IgG1
Specificity Actin, cytoplasmic 1
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human,mouse,rat.
Gene Name: actin, beta
Protein Name: Actin, cytoplasmic 1
Purification Ascites
Plasmids, Primers & others Plasmids, Primers & others beta Actin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name ACTB (ACTB Antibody Abstract)
Background The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Synonyms: BRWS1|PS1TP5BP1|BETA-ACTIN|ACTIN, CYTOPLASMIC, 1
Gene ID 60, 11461, 81822
UniProt P60709, P60710, P60711
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Application Notes WB: Concentration: 0.25-0.5 μg/mLμg/mL, Species: Human, mouse, rat
IHC-P: Concentration: 0.5-1 μg/mL, Species: Human, mouse, rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Ovary Tissue Lysa...
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Lung Cancer Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Mouse Intestine Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Rat Intestine Tissue
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Liver Tissue Lysa...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Ovary Tissue Lysa...
Product cited in: Cui, Ma, Yu, Jiang, Fu, Fu, Yu, Huang, Hou, Si, Chen, Yu, Wan, Wang: "Autologous Bone Marrow Mononuclear Cell Transplantation Delays Progression of Carotid Atherosclerosis in Rabbits." in: Molecular neurobiology, Vol. 53, Issue 7, pp. 4387-96, 2018 (PubMed).

Wang, Yan, Ji, Ji, Wang, Guo, Li: "miR-139 Functions as An Antioncomir to Repress Glioma Progression Through Targeting IGF-1 R, AMY-1, and PGC-1β." in: Technology in cancer research & treatment, Vol. 16, Issue 4, pp. 497-511, 2018 (PubMed).

Duan, Yan, Liu, Wang, Li, Hu, Chen: "Target delivery of small interfering RNAs with vitamin E-coupled nanoparticles for treating hepatitis C." in: Scientific reports, Vol. 6, pp. 24867, 2018 (PubMed).

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, 2018 (PubMed).

He, Wang, Huang, Peng, Zheng, Xia, Xu, Tian: "The ORF3 Protein of Genotype 1 Hepatitis E Virus Suppresses TLR3-induced NF-κB Signaling via TRADD and RIP1." in: Scientific reports, Vol. 6, pp. 27597, 2018 (PubMed).

Zhang, Wu, Zhang, Shang, Fang, Zhuang: "Fibrotic microenvironment promotes the metastatic seeding of tumor cells via activating the fibronectin 1/secreted phosphoprotein 1-integrin signaling." in: Oncotarget, Vol. 7, Issue 29, pp. 45702-45714, 2018 (PubMed).

Cheng, Tian, Wang, Qi, Huang, Li, Chen: "Downregulation of HP1α suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression." in: Oncotarget, Vol. 7, Issue 30, pp. 48107-48119, 2018 (PubMed).

Wang, Lu, You, Huang, Chen, Liu: "The mTOR/AP-1/VEGF signaling pathway regulates vascular endothelial cell growth." in: Oncotarget, Vol. 7, Issue 33, pp. 53269-53276, 2018 (PubMed).

Wang, Li, Wang, Wang, Yan, Zhang, Ma, Du, Liu, Yang: "MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28." in: Oncotarget, Vol. 7, Issue 43, pp. 70194-70210, 2018 (PubMed).

Zha, Xu, Chen, Yang, Zhang, Lu, Yu, Li, Wu, Zheng, Li: "Generation of V α13/β21+T cell specific target CML cells by TCR gene transfer." in: Oncotarget, Vol. 7, Issue 51, pp. 84246-84257, 2018 (PubMed).

Zhang, Liu, Lian, Liao, Chen, Qin, Wang, Jiang, Wang, Gong: "Monoacylglycerol Lipase: A Novel Potential Therapeutic Target and Prognostic Indicator for Hepatocellular Carcinoma." in: Scientific reports, Vol. 6, pp. 35784, 2018 (PubMed).

Liu, Gao, Wang, Ding, Lv, Gao, Wang, Niu: "Ubenimex attenuates acquired sorafenib resistance in renal cell carcinoma by inhibiting Akt signaling in a lipophagy associated mechanism." in: Oncotarget, Vol. 7, Issue 48, pp. 79141-79153, 2018 (PubMed).

Xiao, Zhang, Li, Wu, Liu, Tan, Du: "Dioscin augments HSV-tk-mediated suicide gene therapy for melanoma by promoting connexin-based intercellular communication." in: Oncotarget, Vol. 8, Issue 1, pp. 798-807, 2018 (PubMed).

Ouyang, Chang, Fang, Qin, Qu, Cheng: "Estrogen-induced SDF-1α production promotes the progression of ER-negative breast cancer via the accumulation of MDSCs in the tumor microenvironment." in: Scientific reports, Vol. 6, pp. 39541, 2018 (PubMed).

Zhao, Xu, Xu, Qi, Li, Cheng, Liu, Wu, Gao, Ye: "The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma." in: Scientific reports, Vol. 6, pp. 38471, 2018 (PubMed).

Li, Guan, Liu, Zhao, Li, Zhang, Tan, Mi, Li: "Epoxyeicosanoids prevent intervertebral disc degeneration in vitro and in vivo." in: Oncotarget, Vol. 8, Issue 3, pp. 3781-3797, 2018 (PubMed).

Liu, Lv, Zhu, Wu, Zhang, Xu, Zheng, Zhao: "Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo." in: Scientific reports, Vol. 7, pp. 39869, 2018 (PubMed).

Li, Fang, Jiang, Wang, Ma, Kang, Zhang, Miao, Wang, Guo, Zhang, Guo, Zhao, Yang, Tian, Xiao: "STAT3 and NF-κB are Simultaneously Suppressed in Dendritic Cells in Lung Cancer." in: Scientific reports, Vol. 7, pp. 45395, 2018 (PubMed).

Duan, Guo, Li, Yuan: "Antifatigue Effect of Luteolin-6-C-Neohesperidoside on Oxidative Stress Injury Induced by Forced Swimming of Rats through Modulation of Nrf2/ARE Signaling Pathways." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 3159358, 2018 (PubMed).

Xie, Yu, Chen, Zhang, Ma, Di: "HMGB1 regulates P-glycoprotein expression in status epilepticus rat brains via the RAGE/NF-κB signaling pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1691-1700, 2018 (PubMed).

Did you look for something else?