beta Actin antibody (Actin, beta) (Cytosolic)

Details for Product anti-ACTB Antibody No. ABIN3044484, Supplier: Log in to see
Antigen
  • BRWS1
  • PS1TP5BP1
  • ACTA1
  • Actx
  • E430023M04Rik
  • beta-actin
  • ps1tp5bp1
  • act-2
  • actb
  • B-actin
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • ACT-5
  • ACTB
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • Beta-actin
  • actin beta
  • actin, beta
  • cytoplasmic actin OlCA1
  • beta-actin
  • Actin 5C
  • actin gamma 1
  • actin, beta 1
  • actin, beta L homeolog
  • beta actin
  • actin, cytoplasmic 1
  • ACTB
  • Actb
  • actb
  • LOC100174883
  • LOC100304816
  • Act5C
  • ACTG1
  • actb1
  • actb.L
  • LOC100136352
  • LOC109073280
Alternatives
anti-Cow (Bovine) beta Actin antibody for Immunocytochemistry
Epitope
Cytosolic, N-Term
149
42
17
17
10
8
7
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
593
347
304
109
86
84
63
58
54
45
42
42
34
32
28
23
16
15
15
15
10
9
8
7
7
6
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
359
259
12
7
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
34
29
19
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
579
252
183
134
114
97
60
45
37
15
14
13
12
9
9
2
2
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Purpose Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human,mouse,rat. No cross reactivity with other proteins.
Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Sequence Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys
Clone AC-15
Isotype IgG1
Specificity Actin, cytoplasmic 1
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human,mouse,rat.
Gene Name: actin, beta
Protein Name: Actin, cytoplasmic 1
Purification Ascites
Plasmids, Primers & others Plasmids, Primers & others beta Actin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name ACTB (ACTB Antibody Abstract)
Background The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Synonyms: BRWS1|PS1TP5BP1|BETA-ACTIN|ACTIN, CYTOPLASMIC, 1
Gene ID 60, 11461, 81822
UniProt P60709, P60710, P60711
Pathways Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Application Notes WB: Concentration: 0.25-0.5 μg/mLμg/mL, Species: Human, mouse, rat
IHC-P: Concentration: 0.5-1 μg/mL, Species: Human, mouse, rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Ovary Tissue Lysa...
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Lung Cancer Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Mouse Intestine Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), IHC(P) IHC(P): Rat Intestine Tissue
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Liver Tissue Lysa...
Western Blotting (WB) image for anti-Actin, beta (ACTB) (Cytosolic), (N-Term) antibody (ABIN3044484) Anti-beta-Actin antibody (monoclonal), Western blotting Lane 1: Rat Ovary Tissue Lysa...
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