TICAM1 antibody (C-Term)

Catalog No. ABIN3044491

Product Details anti-TICAM1 Antibody

Target: TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))

Binding Specificity: AA 692-712, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for TIR domain-containing adapter molecule 1(TICAM1) detection. Tested with WB, IHC-P, IHC-F in Human.

Sequence: NNHMWNQRGS QAPEDKTQEA E

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for TIR domain-containing adapter molecule 1(TICAM1) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: toll-like receptor adaptor molecule 1
Protein Name: TIR domain-containing adapter molecule 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRIF(692-712aa NNHMWNQRGSQAPEDKTQEAE).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for TICAM1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-TICAM1 antibody (ABIN3044491)

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Target Details for TICAM1

Target: TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))

Alternative Name: TICAM1 (TICAM1 Products)

Synonyms: IIAE6 antibody, MyD88-3 antibody, PRVTIRB antibody, TICAM-1 antibody, TRIF antibody, AW046014 antibody, AW547018 antibody, trif antibody, toll like receptor adaptor molecule 1 antibody, toll-like receptor adaptor molecule 1 antibody, TICAM1 antibody, Ticam1 antibody, ticam1 antibody

Background: TICAM(TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TICAM1 or TRIF, is an adapter in responding to activation of toll-like receptors(TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al.(2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al.(2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering(si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.

Synonyms: HSD34 antibody|MGC35334 antibody|Proline rich vinculin and TIR domain containing protein B antibody|Proline-rich antibody|PRVTIRB antibody|Putative NF kappa B activating protein 502H antibody|Putative NF-kappa-B-activating protein 502H antibody|Putative NFkB activating protein antibody|Ring finger protein 36 antibody|RNF36 antibody|TCAM1_HUMAN antibody|TICAM 1 antibody|TICAM-1 antibody|TICAM1 antibody|TIR domain containing adapter molecule 1 antibody|TIR domain containing adapter protein inducing IFN beta antibody|TIR domain containing adaptor inducing interferon beta antibody|TIR domain-containing adapter molecule 1 antibody|TIR domain-containing adapter protein inducing IFN-beta antibody|Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody|Toll like receptor adaptor molecule 1 antibody|Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody|TRIF antibody|TRIF protein antibody|TRIM 69 antibody|TRIM69 antibody|Trimless antibody|Tripartite motif containing 69 antibody|vinculin and TIR domain-containing protein B antibody

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades