TNFSF8 antibody (AA 63-234)

Catalog No. ABIN3044499

Product Details anti-TNFSF8 Antibody

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Binding Specificity: AA 63-234

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFSF8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 8(TNFSF8) detection. Tested with IHC-P, FCM in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 8(TNFSF8) detection. Tested with IHC-P, FCM in Human.
Gene Name: tumor necrosis factor superfamily member 8
Protein Name: Tumor necrosis factor ligand superfamily member 8

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.

Isotype: IgG

Application Details

Application Notes: IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for TNFSF8

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Alternative Name: TNFSF8 (TNFSF8 Products)

Synonyms: CD153 antibody, CD30LG antibody, Cd30l antibody, CD30L antibody, TNFRSF8 antibody, TNFSF8 antibody, tumor necrosis factor (ligand) superfamily, member 8 antibody, TNF superfamily member 8 antibody, tumor necrosis factor superfamily member 8 antibody, Tnfsf8 antibody, TNFSF8 antibody

Background: CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms: CD153 antibody|CD153 antigen antibody|CD30 antigen ligand antibody|CD30 L antibody|CD30 ligand antibody|CD30-L antibody|CD30L antibody|CD30LG antibody|MGC138144 antibody|TNFL8_HUMAN antibody|Tnfsf8 antibody|Tumor necrosis factor (ligand) superfamily member 8 antibody|Tumor necrosis factor ligand superfamily member 8 antibody

Gene ID: 944

UniProt: P32971