UBA1 antibody (N-Term)

Catalog No. ABIN3044558

Product Details anti-UBA1 Antibody

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Binding Specificity: AA 102-139, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Ubiquitin-like modifier-activating enzyme 1(UBA1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: HDQGTAQWAD LSSQFYLREE DIGKNRAEVS QPRLAELN

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Ubiquitin-like modifier-activating enzyme 1(UBA1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ubiquitin like modifier activating enzyme 1
Protein Name: Ubiquitin-like modifier-activating enzyme 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human UBA1 (102-139aa HDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELN), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: UBA1 (UBA1 Products)

Synonyms: A1S9 antibody, A1S9T antibody, A1ST antibody, AMCX1 antibody, GXP1 antibody, POC20 antibody, SMAX2 antibody, UBA1A antibody, UBE1 antibody, UBE1X antibody, DDBDRAFT_0190927 antibody, DDBDRAFT_0220497 antibody, DDB_0190927 antibody, DDB_0220497 antibody, Sbx antibody, Ube-1 antibody, Ube1x antibody, a1s9 antibody, a1s9t antibody, a1st antibody, amcx1 antibody, gxp1 antibody, poc20 antibody, smax2 antibody, uba1a antibody, ube1 antibody, ube1x antibody, uba1 antibody, uba1b antibody, ube antibody, zgc:66143 antibody, wu:fa01e08 antibody, wu:fb30f01 antibody, wu:fi21c11 antibody, wu:fj14g11 antibody, ubiquitin like modifier activating enzyme 1 antibody, ubiquitin activating enzyme E1 antibody, Ubiquitin-conjugating enzyme E1 antibody, ubiquitin-like modifier activating enzyme 1 antibody, ubiquitin-like modifier activating enzyme 1 S homeolog antibody, ubiquitin-like modifier activating enzyme 1 L homeolog antibody, UBA1 antibody, uae1 antibody, GL50803_4083 antibody, GL50803_10661 antibody, Uba1 antibody, uba1.S antibody, uba1.L antibody, uba1 antibody, ptr3 antibody

Background: Ubiquitin-like modifier activating enzyme 1 (UBA1) is an enzyme which in humans is encoded by the UBA1 gene. The protein encoded by this gene catalyzes the first step in ubiquitin conjugation, or ubiquitination, to mark cellular proteins for degradation. Specifically, UBA1 catalyzes the ATP-dependent adenylation of ubiquitin (Ub), thereby forming a thioester bond between the two. It also continues to participate in subsequent steps of ubiquination as a Ub carrier. UBA1 is one of only two human ubiquitin-activating enzymes (E1), the other being UBA6, and thus is largely responsible for protein ubiquitination in humans. Through its central role in ubiquitination, UBA1 has been linked to cell cycle regulation, endocytosis, signal transduction, apoptosis, DNA damage repair, and transcriptional regulation. Additionally, UBE1 helps regulate the NEDD8 pathway, thus implicating it in protein folding, as well as mitigating the depletion of ubiquitin levels during stress.

Synonyms: A1S9 antibody|A1S9 protein antibody|A1S9T and BN75 temperature sensitivity complementing antibody|A1S9T antibody|A1ST antibody|AMCX1 antibody|CFAP124 antibody|CTD-2522E6.1 antibody|GXP 1 antibody|GXP1 antibody|MGC4781 antibody|POC20 antibody|POC20 centriolar protein homolog antibody|Protein A1S9 antibody|SMAX2 antibody|Uba1 antibody|UBA1, ubiquitin-activating enzyme E1 homolog A antibody|UBA1_HUMAN antibody|UBA1A antibody|UBE 1 antibody|UBE 1X antibody|UBE1 antibody|UBE1X antibody|Ubiquitin activating enzyme E1 antibody| Ubiquitin-activating enzyme E1 antibody|Ubiquitin-like modifier-activating enzyme 1 antibody

Gene ID: 7317

UniProt: P22314