ZBTB7A antibody (N-Term)

Catalog No. ABIN3044569

This Rabbit Polyclonal antibody specifically detects ZBTB7A in WB and IHC. It exhibits reactivity toward Mouse and Rat.

Quick Overview for ZBTB7A antibody (N-Term) (ABIN3044569)

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB7A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ZBTB7A Antibody

Binding Specificity: AA 125-163, N-Term

Purpose: Anti-ZBTB7A Antibody Picoband®

Sequence: DLLERQILAA DDVGDASQPD GAGPTDQRNL LRAKEYLEF

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ZBTB7A Antibody Picoband® (ABIN3044569). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse ZBTB7A, different from the related human sequence by eleven amino acids, and from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Mouse
1. "Entrez Gene: ZBTB7A zinc finger and BTB domain containing 7A". 2. Wang, G., Lunardi, A., Zhang, J., Chen, Z., Ala, U., Webster, K. A., Tay, Y., Gonzalez-Billalabeitia, E., Egia, A., Shaffer, D. R., Carver, B., Liu, X.-S., Taulli, R., Kuo, W. P., Nardella, C., Signoretti, S., Cordon-Cardo, C., Gerald, W. L., Pandolfi, P. P. Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion. Nature Genet. 45: 739-746, 2013.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZBTB7A

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Alternative Name: Zbtb7a

Background:

Synonyms: Zinc finger and BTB domain-containing protein 7A,Leukemia/lymphoma-related factor,POZ and Krueppel erythroid myeloid ontogenic factor,POK erythroid myeloid ontogenic factor,Pokemon,Zbtb7a,Lrf, Zbtb7,

Tissue Specificity: Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In the spleen, mainly expressed in the white pulp germinal centers (at protein level). Up-regulated in thymic lymphomas. .

Background: Zinc finger and BTB domain-containing protein 7A is a protein that in humans is encoded by the ZBTB7A gene. ZBTB7A has a critical oncosuppressive role in the prostate. Prostate-specific inactivation of ZBTB7A leads to a marked acceleration of PTEN loss-driven prostate tumorigenesis through bypass of PTEN loss-induced cellular senescence. It has been showed that ZBTB7A physically interacts with SOX9 and functionally antagonizes its transcriptional activity on key target genes such as MIA, which is involved in tumor cell invasion, and H19, a long noncoding RNA precursor for an RB-targeting microRNA. Inactivation of ZBTB7A in vivo leads to RB downregulation, bypass of PTEN loss-induced cellular senescence, and invasive prostate cancer. Notably, it has been also found that ZBTB7A is genetically lost, as well as downregulated at both the mRNA and protein levels, in a subset of human advanced prostate cancers. Therefore, ZBTB7A is identified as a context-dependent cancer gene that can act as an oncogene in some contexts but that also has oncosuppressive-like activity in PTEN-null tumors.

Molecular Weight: 75 kDa

Gene ID: 16969

UniProt: O88939