F4/80 antibody

Catalog No. ABIN3044608

Product Details anti-F4/80 Antibody

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Rat monoclonal Anti-Mouse F4/80 is recommended for use in flow cytometry for identification of F4/80 glycoprotein, present in macrophages.

Immunogen: BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.

Clone: BM8

Isotype: IgG2a

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis usingless than or equal to 1 µg/10^6 cells.

Comment:

Purified, unconjugated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: F4/80 (EMR1 Products)

Synonyms: EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody

Background: F4/80 is a 160 kDa glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8 T cells- mediated peripheral tolerance.