Donkey anti-Mouse IgG (Whole Molecule) Antibody (TRITC)

Catalog No. ABIN3045787

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Whole Molecule

Reactivity: Mouse

Host: Donkey

Conjugate: TRITC

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Specificity: Based on IEP, this antibody reacts with:
heavy (γ) chains on mouse IgG
light chains on all mouse immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to:
non-immunoglobulin mouse serum immunoglobulins

Characteristics: Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Purification: Affinity purified using solid phase Mouse IgG

Purity: > 90 % based on SDS-PAGE Small amounts of intact IgG may be present.

Immunogen: Purified Mouse IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator.
Suggested starting dilution(s): • 1:20 - 1:2,000 for most applications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8 °C
Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody