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Donkey anti-Sheep IgG (Whole Molecule) Antibody (DyLight 488)

FACS, IM DyLight 488
Catalog No. ABIN3045811
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2333
    • 499
    • 411
    • 187
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Whole Molecule
    Reactivity
    • 692
    • 672
    • 559
    • 452
    • 366
    • 239
    • 197
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Sheep
    Host
    • 1718
    • 1474
    • 536
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Donkey
    Conjugate
    • 556
    • 408
    • 406
    • 367
    • 298
    • 276
    • 228
    • 119
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 31
    • 31
    • 30
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    DyLight 488
    Application
    • 1837
    • 1789
    • 1786
    • 1499
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 99
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunomicroscopy (IM)
    Specificity
    Based on IEP, this antibody reacts with:
    heavy (γ) chains on sheep IgG
    light chains on all sheep immunoglobulins
    Cross-Reactivity (Details)
    Based on IEP, no reactivity is observed to:
    non-immunoglobulin sheep serum proteins
    Characteristics
    Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
    Purification
    Affinity purified using solid phase Sheep IgG
    Purity
    > 95 % based on SDS-PAGE
    Immunogen
    Purified Sheep IgG, whole molecule
  • Application Notes
    This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator.
    Suggested starting dilution(s): • 1:20 - 1:2,000 for most applications
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
    Concentration
    1 mg/mL
    Buffer
    10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
    0.05 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store freeze-dried powder at 2-8 °C.
    Shelf Life: Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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