PPAP2B antibody

Catalog No. ABIN306894

Product Details anti-PPAP2B Antibody

Target: PPAP2B (Phosphatidic Acid Phosphatase Type 2B (PPAP2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAP2B antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Lipid phosphate phosphohydrolase 3, EC 3.1.3.4 Phosphatidic acid phosphatase 2b, PAP2-beta ,PAP-2b, PAP2b, Phosphatidate phosphohydrolase type 2b, Vascular endothelial growth factor and type I collagen-inducible protein, VCIP,Phosphatidic acid phosphatase type 2 (PAP2) was originally identified as a plasma membrane enzyme that catalyses the dephosphorylation of the putative second messenger, phosphatidic acid (PA) to diacylglycerol (DG) [1]. Subsequently, multiple isoforms of PAP2 were cloned [2-5]. It was found that these enzymes dephosphorylate a number of lipid phosphates in vitro other than PA, including the potent bioactive lipids, lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P). Therefore, they have been renamed lipid phosphate phosphatases (LPPs). Currently, there are four members of this family called LPP1, LPP1a, LPP2 and LPP3 [6]. S1P [7] and LPA [8] regulate the proliferation, differentiation, apoptosis and migration of cells by binding to a family of G protein-coupled receptors. Thus, EDG1/S1P1, EDG3/S1P3, EDG5/S1P2/AGR16/H218, EDG6/S1P4 and EDG8/S1P5/nrg-1 are high affinity S1P receptors [7] whereas EDG2/LPA1, EDG4/LPA2 and EDG7/LPA3 have high affinity for LPA [8]. Recently, the over-expression of LPP1 was shown to limit LPA-stimulated signalling in Rat2 fibroblasts [9] and LPA-stimulated DNA synthesis in HEK 293 cells [10]. Similarly, over-expression of LPP1, LPP1a and LPP2 attenuate S1P-signalling to the p42/p44 mitogen activated protein kinase cascade [11].

Purification: Ammonium Sulfate Precipitation

Immunogen: Rabbits were immunized with a unique peptide derived from the human lysophospholipid phosphatase 3 protein.

Isotype: IgG

Application Details

Application Notes: This antibody can be used for Western blotting (10-15 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for PPAP2B

Target: PPAP2B (Phosphatidic Acid Phosphatase Type 2B (PPAP2B))

Alternative Name: Lyosphospholipid Phosphatase (LPP) 3 (PPAP2B Products)

Synonyms: Dri42 antibody, LPP3 antibody, PAP2B antibody, VCIP antibody, 1110003O22Rik antibody, 2610002D05Rik antibody, AV025606 antibody, D4Bwg0538e antibody, D4Bwg1535e antibody, Lpp3 antibody, Ppab2b antibody, dri42 antibody, lpp3 antibody, pap2b antibody, ppap2bb antibody, vcip antibody, ppap2b antibody, ppap2ba antibody, PPAP2B antibody, si:dkey-19f4.1 antibody, phospholipid phosphatase 3 antibody, phospholipid phosphatase 3 L homeolog antibody, phospholipid phosphatase 3 S homeolog antibody, PLPP3 antibody, Plpp3 antibody, plpp3.L antibody, plpp3.S antibody, plpp3 antibody