NPDC1 antibody

Catalog No. ABIN306963

Product Details anti-NPDC1 Antibody

Target: NPDC1 (Neural Proliferation, Differentiation and Control, 1 (NPDC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPDC1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Monoclonal Anti-NPDC-1 (mouse IgG1 isotype) is derived from the hybridoma 16E4 produced by immunizing mice with the peptide SLGSPVSSDPVHMS (Gene ID: 56654).

Cross-Reactivity: Human, Mouse, Rat

Characteristics: The discovery of NPDC-1 (neuronal proliferation, differentiation and control protein 1) was the result of a study of genes that were preferentially expressed in immortalized neural precursor cell lines at the onset of contact inhibition of proliferation and subsequent differentiation. It was further found that transfection of transformed cells with NPDC-1 led to increased doubling times and suppression of many characteristics of transformation. NPDC-1 is differentially expressed in brain and lung and has been shown to interact directly with the transcription factor E2F-1 as well as D-type cyclins and cdk2. NPDC-1 has been identified as a modulator of transcriptional events mediated by retinoic acid and the protein level and activity of NPDC-1 have been shown to be regulated by phosphorylation mediated proteasomal degradation.

Purification: Ion-exchange chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide SLGSPVSSDPVHMS derived from human NPDC-1 protein (Gene ID: 56654) and mouse myeloma cells.

Clone: 16E4

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions. Monoclonal Anti-NPDC-1 recognizes human NPDC-1 by immunoblotting (34-40 kDa).

Restrictions: For Research Use only

Handling

Buffer: 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol, pH 7.5, 3 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for NPDC1

Target: NPDC1 (Neural Proliferation, Differentiation and Control, 1 (NPDC1))

Alternative Name: NPDC-1 (NPDC1 Products)

Synonyms: AI314472 antibody, NPDC-1 antibody, CAB antibody, CAB- antibody, CAB-1 antibody, CAB1 antibody, neural proliferation, differentiation and control, 1, gene 1 L homeolog antibody, neural proliferation, differentiation and control 1 antibody, neural proliferation, differentiation and control, 1 antibody, npdc1.1.L antibody, Npdc1 antibody, NPDC1 antibody

UniProt: Q8WXX4