M13 Bacteriophage (g3p) antibody (Biotin)
Reactivity: Mouse
FACS, IHC, ELISA, WB
Host: Mouse
Monoclonal
E1
Biotin
1 image
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- Target
- M13 Bacteriophage (g3p)
- Reactivity
- Mouse
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Biotin
- Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- Characteristics
- The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
- Purification
- Protein A/G Chromatography
- Immunogen
- M13 phage coat proteins.,Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.
- Clone
- E1
- Isotype
- IgG3
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- Application Notes
- Antibody can be used for immunohistochemistry, Western blot (1-5 μg/mL), Flow cytometry (1 μg/10^6 cells) and ELISA. Optimal concentration should be evaluated by serial dilutions.
- Restrictions
- For Research Use only
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- Buffer
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide,Antibody specific for the M13 bacteriophage protein coat, amongst others the g3p protein.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to avoid freeze/thaw cycles
- Storage
- -20 °C
- Storage Comment
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
- Expiry Date
- 12 months
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- Target
- M13 Bacteriophage (g3p)
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