SRPRB antibody (AA 246-265)

Catalog No. ABIN307164

Product Details anti-SRPRB Antibody

Target: SRPRB (Signal Recognition Particle Receptor, B Subunit (SRPRB))

Binding Specificity: AA 246-265

Reactivity: Dog

Host: Sheep

Clonality: Polyclonal

Conjugate: This SRPRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Chicken, Dog

Characteristics: Signal Recognition Particle Receptor, β subunit,The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide corresponding to amino acids 246-265 derived from the carboxyl terminal of the canine SRbeta subunit.

Isotype: IgG

Application Details

Application Notes: Detects SRbeta by Western blot analysis at 15 μg/mL. Antibody will not detect SRbeta protein bound to SRalpha. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SRPRB

Target: SRPRB (Signal Recognition Particle Receptor, B Subunit (SRPRB))

Alternative Name: SR beta (SRPRB Products)

Synonyms: wu:fk57h10 antibody, zgc:111820 antibody, zgc:92746 antibody, apmcf1 antibody, APMCF1 antibody, AA409543 antibody, Ab2-417 antibody, Ba1-667 antibody, Cc1-8 antibody, signal recognition particle receptor, B subunit antibody, SRP receptor beta subunit S homeolog antibody, SRP receptor beta subunit antibody, SRPRB antibody, srprb antibody, srprb.S antibody, Srprb antibody

Pathways: ER-Nucleus Signaling