MARCH10 antibody (Internal Region)

Catalog No. ABIN308396

Product Details anti-MARCH10 Antibody

Target: MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MARCH10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MARCH10

Sequence: SRNYPQPRTE E

Specificity: This antibody is expected to recognise both reported isoforms (NP_001094345.1, NP_689811.2), which are identical.

Cross-Reactivity: Cow, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1, NP_689811.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. An additiona

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MARCH10

Target: MARCH10 (Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))

Alternative Name: MARCH10 (MARCH10 Products)

Synonyms: 4933417C16Rik antibody, AV039223 antibody, OTTMUSG00000002847 antibody, Rnf190 antibody, MARCH-X antibody, RGD1311692 antibody, RNF190 antibody, membrane associated ring-CH-type finger 10 antibody, membrane-associated ring finger (C3HC4) 10 antibody, MARCH10 antibody, March10 antibody

Background: MARCH10, membrane-associated ring finger (C3HC4) 10 , FLJ35757, MARCH-X, RNF190, ring finger protein 190

Gene ID: 162333, 303596

NCBI Accession: NP_001094345, NP_689811