Pleckstrin Homology Domain Containing, Family B (Evectins) Member 1 (PLEKHB1) (N-Term) antibody

Details for Product No. ABIN308463
Antigen
Epitope
N-Term
7
1
Reactivity
Rat (Rattus)
8
3
2
1
1
1
1
1
Host
Goat
6
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
9
8
Options
Purpose PHR1
Immunogen Peptide with sequence PPDSALESPFEE-C, from the N Terminus of the protein sequence according to NP_067023.1, NP_001123506.1, NP_001123505.1, NP_001123507.1, NP_001123508.1,.
Blocking Peptide Blocking peptide for this product available: ABIN368728
Sequence PPDSALESPF EE
Isotype IgG
Specificity This antibody is expected to recognize reported isoforms a (NP_067023.1) and c (NP_001123505.1).
Cross-Reactivity Human, Mouse (Murine), Cow (Bovine)
Predicted Reactivity Human, Mouse, Rat, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name PLEKHB1 (PLEKHB1 Antibody Abstract)
Background PLEKHB1, pleckstrin homology domain containing, family B (evectins) member 1, KPL1, PHR1, PHRET1, PH domain containing protein in retina 1, PH domain containing, retinal 1, evectin-1
Gene ID 58473
NCBI Accession NP_067023, NP_001123506, NP_001123505, NP_001123507, NP_001123508
Application Notes

DS WB Results: Approx. 25 kDa band observed in Rat Brain lysates (calculated MW of 25.2 kDa according to Rat NP_742030.1 (Evectin-1) and of 27.2 kDa according to Human NP_067023.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Pleckstrin Homology Domain Containing, Family B (Evectins) Member 1 (PLEKHB1) (N-Term) antibody (ABIN308463) ABIN308463 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Prima...