MARCKS antibody (Myristoylated Alanine-Rich Protein Kinase C Substrate) (Ser152)

Details for Product anti-MARCKS Antibody No. ABIN3170501
Antigen
Epitope
Ser152, Ser156
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28
27
24
19
15
11
10
8
5
5
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4
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3
3
3
2
2
2
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2
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
239
155
152
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16
15
7
5
3
3
2
1
1
1
Host
Rabbit
238
10
3
Clonality
Polyclonal
Conjugate
This MARCKS antibody is un-conjugated
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7
7
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7
7
7
4
4
2
2
2
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2
2
Application
Western Blotting (WB)
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83
47
42
27
26
11
6
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1
Options
Immunogen Synthetic peptide corresponding to AA surrounding Ser152,Ser156 of rat MARCKS
Isotype IgG
Specificity The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.
Purification Affinity purified
Antigen
Alternative Name MARCKS (MARCKS Antibody Abstract)
Background MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
Synonyms: MACS , PRKCSL , MRACKS , PKCSL , FLJ14368 , FLJ90045 , phosphomyristin
Gene ID 266495
UniProt P30009
Application Notes Western blotting (1:200-1000). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μL affinity purified rabbit anti-phospho-MARCKS polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months