5-Hydroxytryptamine (5-HT) (AA 251-266) antibody

Details for Product No. ABIN3170511
Antigen
Epitope
AA 251-266
1
Reactivity
Chemical
33
21
19
18
11
5
4
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
75
12
6
4
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
61
34
23
22
17
13
11
7
4
3
2
2
1
1
1
1
1
Options
Purpose Polyclonal antibody against EDG7
Immunogen Peptide KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine Receptor 1A (Accession P19327). 3rd intracellular loop.Will not recognize human 5-Hydroxytryptamine Receptor 1A.
Sequence KKSLNGQPGS GDWRRC
Isotype IgG
Characteristics Polyclonal antibody against EDG7
Purification Affinity purified
Antigen
Alternative Name Serotonin
Target Type Chemical
Background Serotonin [5-hydroxytryptamine (5-HT1)] is an important neurotransmitter in the CNS that exerts its neuronal activity via 5-HT receptors. 5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1 The 5-HT receptor families belong to the G-Protein Coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels. The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1e AND 5-HT1F) which share 41-63 % overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation. The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the central nervous system, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species. Activation of 5-HT1A receptor decreases blood pressure and heart rate, involved in learning, memory, relieving anxiety and depression. 5-Hydroxytryptamine Receptor
Synonyms: 5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, Serotonin receptor 1A, Htr1a, 5ht1a
Gene ID 24473
UniProt P19327
Application Notes WB: 1:200-1:2000, IHC: 1:100
Comment

Suggested positive control:
Western blot, IHC

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 100 μg (0.5 mg/mL) of antibody in PBS, pH 7.4, 1 % BSA, 0.05 % Sodium azide.
Handling Advice The antibody solution should be gently mixed before use
Storage -20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-5-Hydroxytryptamine (5-HT) (AA 251-266) antibody (ABIN3170511) Western blot analysis of mouse brain membrane (Ianes 1 and 3) and rat brain membrane ...
Image no. 2 for anti-5-Hydroxytryptamine (5-HT) (AA 251-266) antibody (ABIN3170511) Expression of 5-HT1A in rat cerebellum Immunohistochemical staining of rat cerebellum...