SIRPA antibody (FITC)

Catalog No. ABIN317417

Product Details anti-SIRPA Antibody (FITC)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Characteristics: Synonyms: BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, P84, SHPS-1, Sirp-alpha-1, Sirp-alpha-2,Sirp-alpha-3, MyD-1, SHP Substrate 1, Tyrosine-Protein Phosphatase Non-Receptor TypeSubstrate 1, Inhibitory receptor SHPS-1, Signal-Regulatory Protein Alpha-1, Brain Ig-likeMolecule with Tyrosine-based Activation Motifs, Macrophage Fusion Receptor

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Porcine alveolar macrophages

Clone: BL1H7

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use 10 μL of neat - 1/10 diluted antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. This aproduct is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a / SIRPA (SIRPA Products)

Synonyms: BIT antibody, CD172A antibody, MFR antibody, MYD-1 antibody, P84 antibody, PTPNS1 antibody, SHPS1 antibody, SIRP antibody, SIRPA antibody, SIRP-A1 antibody, SIRP-B1 antibody, AI835480 antibody, Bit antibody, CD172a antibody, Ptpns1 antibody, SHP-1 antibody, SHPS-1 antibody, swc3 antibody, signal regulatory protein alpha antibody, myeloperoxidase antibody, signal-regulatory protein alpha antibody, tyrosine-protein phosphatase non-receptor type substrate 1 antibody, SIRPA antibody, MPO antibody, LOC458039 antibody, LOC100400378 antibody, LOC100590224 antibody, Sirpa antibody

Background: Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84