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PRKACA antibody

PRKACA Reactivity: Human, Mouse, Rat WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN317665
  • Target See all PRKACA Antibodies
    PRKACA (Protein Kinase A, alpha (PRKACA))
    Reactivity
    • 57
    • 38
    • 14
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Mouse, Rat
    Host
    • 60
    • 10
    • 2
    • 1
    Rabbit
    Clonality
    • 63
    • 10
    Polyclonal
    Conjugate
    • 42
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PRKACA antibody is un-conjugated
    Application
    • 58
    • 31
    • 17
    • 13
    • 13
    • 13
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Specificity
    This antibody detects endogenous levels of PKAalpha/beta cat protein.(region surrounding Lys17)
    Purification
    Affinity Chromatography using epitope-specific immunogen.
    Top Product
    Discover our top product PRKACA Primary Antibody
  • Application Notes
    ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    PRKACA (Protein Kinase A, alpha (PRKACA))
    Alternative Name
    PRKACA (PRKACA Products)
    Synonyms
    PKACA antibody, Calpha antibody, Cs-PKA antibody, PKCA1 antibody, PKA antibody, PKCD antibody, Pkaca antibody, zgc:158799 antibody, protein kinase cAMP-activated catalytic subunit alpha antibody, protein kinase, cAMP dependent, catalytic, alpha antibody, cAMP-dependent protein kinase catalytic subunit antibody, protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a antibody, PRKACA antibody, Prkaca antibody, kin-1 antibody, prkacaa antibody
    Background
    The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93 % amino acid sequence similarity), whereas Cgamma displays 83 % and 79 % similarity to Calpha and Cbeta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
    Molecular Weight
    approx. 38 kDa
    Gene ID
    5566
    NCBI Accession
    NP_002721
    UniProt
    P17612
    Pathways
    NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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