ARFIP1 antibody

Catalog No. ABIN317818

Product Details anti-ARFIP1 Antibody

Target: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Arfaptin-1 protein.(region surrounding Lys314)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARFIP1

Target: ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))

Alternative Name: Arfaptin-1 (ARFIP1 Products)

Synonyms: arfip1 antibody, MGC53116 antibody, DKFZp469C2322 antibody, HSU52521 antibody, 2310030I10Rik antibody, AW123087 antibody, Arfaptin antibody, D3Ertd598e antibody, ADP ribosylation factor interacting protein 1 antibody, ADP ribosylation factor interacting protein 1 L homeolog antibody, Arfaptin-1 antibody, ADP-ribosylation factor interacting protein 1 antibody, ARFIP1 antibody, arfip1.L antibody, arfp1 antibody, Arfip1 antibody

Background: Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.Synonyms: ADP-ribosylation factor-interacting protein 1, ARFIP1

Molecular Weight: approx. 40 kDa

Gene ID: 27236

NCBI Accession: NP_001020764

UniProt: P53367