DCT antibody

Catalog No. ABIN317917

Product Details anti-DCT Antibody

Target: DCT (Dopachrome Tautomerase (DCT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: TRP2 antibody detects endogenous levels of TRP2 protein.(region surrounding Lys89)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DCT

Target: DCT (Dopachrome Tautomerase (DCT))

Alternative Name: TYRP2 / DCT (DCT Products)

Synonyms: TYRP2 antibody, RGD1564975 antibody, trp2 antibody, DT antibody, TRP-2 antibody, TRP2 antibody, Tyrp-2 antibody, Tyrp2 antibody, slaty antibody, slt antibody, zgc:109860 antibody, dopachrome tautomerase antibody, dopachrome tautomerase L homeolog antibody, DCT antibody, Dct antibody, dct antibody, dct.L antibody

Background: Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme,is involved in the production of melanin, the primary pigment found in vertebrates.Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteinerich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exist as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.Synonyms: L-dopachrome Delta-isomerase, L-dopachrome tautomerase, TRP-2, TRP2, Tyrosinase-related protein 2

Molecular Weight: approx. 60 kDa

Gene ID: 1638

NCBI Accession: NP_001913

UniProt: P40126