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CD84 antibody (Internal Region)

CD84 Reactivity: Human, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3187786
  • Target See all CD84 Antibodies
    CD84
    Binding Specificity
    • 16
    • 9
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 63
    • 22
    • 16
    • 1
    Human, Mouse
    Host
    • 49
    • 30
    • 1
    Rabbit
    Clonality
    • 49
    • 30
    • 1
    Polyclonal
    Conjugate
    • 31
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD84 antibody is un-conjugated
    Application
    • 46
    • 43
    • 24
    • 10
    • 9
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    CD84 Polyclonal Antibody detects endogenous levels of CD84 protein.
    Characteristics
    Rabbit Polyclonal to CD84.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    Synthesized peptide derived from the Internal region of human CD84.
    Isotype
    IgG
  • Application Notes
    WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000,
    Restrictions
    For Research Use only
  • Validation #104537 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    TRON
    No.
    #104537
    Date
    12/07/2023
    Antigen
    CD84
    Lot Number
    15601601
    Method validated
    Immunohistochemistry
    Positive Control

    FFPE human tonsil

    Negative Control
    Notes

    Passed. The CD84 antibody ABIN3187786 specifically stains cells within the germinal zone in human tonsil.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3187786
    Secondary Antibody
    HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)
    Full Protocol
    • Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
      • xylene 2x for 5 min.
      • 100 % (v/v) ethanol 2x for 5 min.
      • 96 % (v/v) ethanol 2x for 5 min.
      • 70 % (v/v) ethanol 2x for 5 min.
    • Wash slides 2x 5 min with ddH2O.
    • Cook slides for 30 min at 95 °C in citrate buffer pH 6.
    • Wash slides 2x 3 min with ddH2O.
    • Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
    • Wash slides 3x 3 min in 1X PBS.
    • Block slides in 10 % goat serum in PBS for 30 min at RT.
    • Incubate slides with primary rabbit anti-CD84 antibody (antibodies-online, ABIN3187786, lot 15601601) diluted 1:100 and 1:200 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
    • Wash slides 3x 3 min in 1X PBS.
    • Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
    • Wash slides 3x 3 min in 1X PBS.
    • HRP development.
    • Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
    • Wash slides for 15 min under running tap water.
    • Dehydrate sections in an ascending alcohol-xylene series:
      • 70% ethanol 2x for 10 sec.
      • 96% ethanol 2x for 10 sec.
      • 100% ethanol 2x for 10 sec.
      • xylene 2x for 10 sec.
    • Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
    • Dry sections.
    • Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.
    Experimental Notes
    • Staining with ABIN3187786 overnight at 1:100 and 1:200 antibody dilution the signal in human tonsil was sharp and localized in the cells within the germinal zone (as expected). Modifying ABIN3187786 incubation conditions to 1 h at RT, the signal in human tonsil was still sharp and localized in the cells within the germinal zone.

    • However, in mouse spleen, the signal was almost absent and very weak. With the modified incubation conditions, the signal in mouse spleen was again almost absent and very weak. With either staining protocol the signal in mouse lung was sporadic and its quality does not allow the possibility of non-specific staining to be excluded.

  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C.
  • Target
    CD84
    Alternative Name
    CD84 (CD84 Products)
    Synonyms
    RGD1564553 antibody, A130013D22Rik antibody, CDw84 antibody, SLAMF5 antibody, LY9B antibody, hCD84 antibody, mCD84 antibody, CD84 molecule antibody, CD84 antigen antibody, Cd84 antibody, CD84 antibody
    Molecular Weight
    39 kDa
    Gene ID
    8832
    UniProt
    Q9UIB8
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