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Leptin antibody (AA 22-167)

LEP Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated
Catalog No. ABIN3201296
  • Target See all Leptin (LEP) Antibodies
    Leptin (LEP)
    Binding Specificity
    • 25
    • 16
    • 15
    • 15
    • 12
    • 11
    • 10
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 22-167
    Reactivity
    • 120
    • 73
    • 71
    • 12
    • 8
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Rat
    Host
    • 169
    • 23
    • 6
    • 5
    • 2
    Mouse
    Clonality
    • 180
    • 24
    Monoclonal
    Conjugate
    • 101
    • 26
    • 17
    • 9
    • 8
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This Leptin antibody is un-conjugated
    Application
    • 133
    • 85
    • 64
    • 39
    • 39
    • 38
    • 32
    • 30
    • 25
    • 21
    • 16
    • 11
    • 11
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Monoclonal Antibody to Leptin (LEP)
    Specificity
    The antibody is a mouse monoclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
    Purification
    Protein A + Protein G affinity chromatography
    Immunogen
    Recombinant Leptin (LEP) corresdonding to Val22~Cys167 (Accession # P50596)
    Clone
    C2
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product LEP Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Leptin (LEP)
    Alternative Name
    Leptin (LEP Products)
    Synonyms
    ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
    Background
    OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
    Pathways
    JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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