HSP27 antibody (AA 2-205)
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- Target See all HSP27 Antibodies
- HSP27 (Heat Shock Protein 27 (HSP27))
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Binding Specificity
- AA 2-205
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HSP27 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Purpose
- Monoclonal Antibody to Heat Shock Protein 27 (Hsp27)
- Specificity
- The antibody is a mouse monoclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Heat Shock Protein 27 (Hsp27) corresdonding to Thr2~Lys205 with N-terminal His Tag
- Clone
- D2
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product HSP27 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- HSP27 (Heat Shock Protein 27 (HSP27))
- Alternative Name
- Heat Shock Protein 27 (HSP27 Products)
- Background
- HSPB1, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock 27 kDa Protein 1, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,
- Pathways
- VEGF Signaling
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