ITGA2 antibody (AA 74-277)

Catalog No. ABIN3201578

Product Details anti-ITGA2 Antibody

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Binding Specificity: AA 74-277

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Integrin Alpha 2 (ITGa2)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- P SLIDVVVVCD ESNSIYPWDA VKNFLEKFVQ GLDIGPTKTQ VGLIQYANNP RVVFNLNTYK TKEEMIVATS QTSQYGGDLT NTFGAIQYAR KYAYSAASGG RRSATKVMVV VTDGESHDGS MLKAVIDQCN HDNILRFGIA VLGYLNRNAL DTKNLIKEIK AIASIPTERY FFNVSDEAAL LEKAGTLGEQ I

Specificity: The antibody is a mouse monoclonal antibody raised against ITGa2. It has been selected for its ability to recognize ITGa2 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Integrin Alpha 2 (ITGa2) corresdonding to Glu74~Val277 (Accession # P17301)

Clone: 1#

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-ITGA2 antibody (ABIN3201578)

Yun, Guan, Liu, Zhang, Wang, Qi, Cui, Liu, Zhang, Gao, Gao, Li, Gao, Wang: "Integrin αvβ1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection." in: The Journal of biological chemistry, Vol. 291, Issue 28, pp. 14815-25, (2016) (PubMed).

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: Integrin Alpha 2 (ITGA2 Products)

Synonyms: BR antibody, CD49B antibody, GPIa antibody, HPA-5 antibody, VLA-2 antibody, VLAA2 antibody, DX5 antibody, integrin subunit alpha 2 antibody, integrin alpha 2 antibody, ITGA2 antibody, Itga2 antibody

Background: CD49b, BR, GPIa, VLA-2, VLAA2, Platelet membrane glycoprotein Ia, Alpha 2 Subunit Of VLA-2 Receptor, Very Late Activation Proteins 2 Receptor, Collagen receptor, VLA-2 alpha

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex