GCKR antibody (AA 90-286)

Catalog No. ABIN3201594

Product Details anti-GCKR Antibody

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Binding Specificity: AA 90-286

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Glucokinase Regulatory Protein (GKRP)

Sequence: MGHHHHHHSG SEF-V QEVLKEPDGG LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE QTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG PEGLSGSSRM KGGGATKILL ETLLLAAHKT VDQGVV

Specificity: The antibody is a mouse monoclonal antibody raised against GKRP. It has been selected for its ability to recognize GKRP in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Glucokinase Regulatory Protein (GKRP) corresdonding to Val90~Ala286 (Accession # Q07071) with N-terminal His Tag

Clone: 4#

Isotype: IgG1 kappa

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: Glucokinase Regulatory Protein (GCKR Products)

Synonyms: FGQTL5 antibody, GKRP antibody, GLRE antibody, gckr antibody, glucokinase regulator antibody, glucokinase regulatory protein antibody, glucokinase regulator L homeolog antibody, GCKR antibody, Gckr antibody, gckr.L antibody

Background: GCKR, Glucokinase(hexokinase 4)regulator

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus