Supervillin antibody (AA 1385-1525)

Catalog No. ABIN3201726

Product Details anti-Supervillin Antibody

Target: Supervillin (SVIL)

Binding Specificity: AA 1385-1525

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Supervillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Supervillin (SVIL)

Sequence: MGHHHHHHSG SEF-CVYFFW QGRHSTVSEK GTSALMTVEL DEERGAQVQV LQGKEPPCFL QCFQGGMVVH SGRREEEEEN VQSEWRLYCV RGEVPVEGNL LEVACHCSSL RSRTSMVVLN VNKALIYLWH GCKAQAHTKE VGRTAANKIK EQCPL

Specificity: The antibody is a mouse monoclonal antibody raised against SVIL. It has been selected for its ability to recognize SVIL in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Supervillin (SVIL) corresdonding to Cys1385~Leu1525 with N-terminal His Tag

Clone: 8#

Isotype: IgG1 kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Supervillin

Target: Supervillin (SVIL)

Alternative Name: Supervillin (SVIL Products)

Synonyms: sb:cb867 antibody, svil antibody, wu:fd05a09 antibody, SVIL antibody, DKFZp468F0819 antibody, AU024053 antibody, B430302E16Rik antibody, supervillin a antibody, supervillin antibody, supervillin, putative antibody, svila antibody, SVIL antibody, LOC5577073 antibody, CpipJ_CPIJ003700 antibody, Smp_148580 antibody, svil antibody, Svil antibody

Background: P205/p250, Archvillin