SFRP4 antibody (AA 22-346)

Catalog No. ABIN3201774

Product Details anti-SFRP4 Antibody

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Binding Specificity: AA 22-346

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFRP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)

Sequence: MGHHHHHHSG SEF-MMLLEN CLVEKWRDQL SKRSIQWEER LQEQRRTVQD KKKTAGRTSR SNPPKPKGKP PAPKPASPKK NIKTRSAQKR TNPKRV

Specificity: The antibody is a mouse monoclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Secreted Frizzled Related Protein 4 (SFRP4) corresdonding to Ala22~Val346 (Accession # Q6FHJ7)

Clone: 5#

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: Secreted Frizzled Related Protein 4 (SFRP4 Products)

Synonyms: SFRP4 antibody, FRP-4 antibody, FRPHE antibody, sFRP-4 antibody, secreted frizzled related protein 4 antibody, secreted frizzled-related protein 4 antibody, SFRP4 antibody, Sfrp4 antibody

Background: FRP-4, FRPHE, Frizzled protein, human endometrium

Pathways: WNT Signaling