REV1 antibody (AA 301-478)

Catalog No. ABIN3201809

Product Details anti-REV1 Antibody

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Binding Specificity: AA 301-478

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This REV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to REV1 Homolog (REV1)

Sequence: MGHHHHHHSG SEF- NKILKGKAA DIPDSSLWEN PDSAQANGID SVLSRAEIAS CSYEARQLGI KNGMFFGHAK QLCPNLQAVP YDFHAYKEVA QTLYETLASY THNIEAVSCD EALVDITEIL AETKLTPDEF ANAVRMEIKD QTKCAASVGI GSNILLARMA TRKAKPDGQY HLKPEEVDDF IRGQLVTNLP GV

Specificity: The antibody is a mouse monoclonal antibody raised against REV1. It has been selected for its ability to recognize REV1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant REV1 Homolog (REV1) corresdonding to Ser301~Lys478 (Accession # Q9UBZ9)

Clone: 10#

Isotype: IgG2a kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for REV1

Target: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Alternative Name: REV1 Homolog (REV1 Products)

Synonyms: ARABIDOPSIS THALIANA HOMOLOG OF REVERSIONLESS 1 antibody, ATREV1 antibody, K23L20.9 antibody, K23L20_9 antibody, 1110027I23Rik antibody, AU022044 antibody, Rev1l antibody, REV1L antibody, DNA-directed DNA polymerase antibody, HIG1 hypoxia inducible domain family member 1C antibody, REV1, DNA directed polymerase antibody, REV1 antibody, HIGD1C antibody, Rev1 antibody

Background: REV1L, aIBP80, Alpha Integrin Binding Protein 80, Rev1-like terminal deoxycytidyl transferase

Pathways: DNA Damage Repair, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response