SLIT1 antibody (AA 647-835)

Catalog No. ABIN3204490

Product Details anti-SLIT1 Antibody

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Binding Specificity: AA 647-835

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Slit Homolog 1 (Slit1)

Specificity: The antibody is a rabbit polyclonal antibody raised against Slit1. It has been selected for its ability to recognize Slit1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Slit Homolog 1 (Slit1) corresdonding to Asn647~Arg835 (Accession # O75093)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SLIT1

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Alternative Name: Slit Homolog 1 (SLIT1 Products)

Synonyms: Slil1 antibody, mKIAA0813 antibody, MEGF4 antibody, SLIL1 antibody, SLIT-1 antibody, SLIT3 antibody, megf4 antibody, slil1 antibody, slit-1 antibody, SLIT1 antibody, slit3 antibody, slit homolog 1 (Drosophila) antibody, slit guidance ligand 1 antibody, slit guidance ligand 1 S homeolog antibody, slit homolog 1 protein antibody, slit homolog 1b (Drosophila) antibody, slit homolog 1a (Drosophila) antibody, Slit1 antibody, SLIT1 antibody, slit1.S antibody, slit1 antibody, LOC100459557 antibody, slit1b antibody, slit1a antibody

Background: MEGF4, SLIL1, SLIT3, Multiple epidermal growth factor-like domains protein 4

Pathways: Regulation of Cell Size