CD28 antibody (AA 33-174)

Catalog No. ABIN3208171

Product Details anti-CD28 Antibody

Target: CD28

Binding Specificity: AA 33-174

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD28. It has been selected for its ability to recognize CD28 in immunohistochemical staining and western blotting.

Characteristics: The antibody is a rabbit polyclonal antibody raised against CD28. It has been selected for its ability to recognize CD28 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: CD28 (Asp33-Val174)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Store at 4 °C for frequent use. Stored at -20 °C to -80 °C in a manual defrost freezer for one year without detectable loss of activity.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: Cluster Of Differentiation 28 (CD28) (CD28 Products)

Synonyms: Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background: Alternative Names: Tp44, T-Cell-Specific Surface Glycoprotein

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response