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LASS5/6 antibody (Internal Region)

Recent LASS5/6 Reactivity: Cow, Dog, Human, Mouse, Rat ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN334429
  • Target See all LASS5/6 products
    LASS5/6 (LAG1 Homolog, Ceramide Synthase 5/6 (LASS5/6))
    Binding Specificity
    Internal Region
    Reactivity
    Cow, Dog, Human, Mouse, Rat
    Host
    Goat
    Clonality
    Polyclonal
    Application
    ELISA
    Purpose
    LASS5+6 / CerS5+6
    Sequence
    RGKVSKDDRS DVES
    Specificity
    This antibbody is expected to cross-react with the highly similar LASS6.
    Predicted Reactivity
    Human, Mouse, Rat, Dog, Cow
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Recent
    Immunogen
    Peptide with sequence C-RGKVSKDDRSDVES, from the internal region of the protein sequence according to NP_671723.1, NP_982288.1.
    Isotype
    IgG
  • Application Notes

    DS WB Results: Preliminary experiments gave an approx. 60 kDa band in lysates of HEK293, HeLa and HepG2 after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.8 kDa according to NP_671723.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    LASS5/6 (LAG1 Homolog, Ceramide Synthase 5/6 (LASS5/6))
    Alternative Name
    LASS5+6 / CerS5+6 (LASS5/6 Products)
    Synonyms
    LASS5 antibody, lass5 antibody, trh4 antibody, ceramide synthase 5 antibody, CERS5 antibody, cers5 antibody
    Background
    LASS5, LAG1 homolog, ceramide synthase 5, CerS5, FLJ25304, MGC45411, Trh4, LAG1 longevity assurance homolog 5
    Gene ID
    91012, 253782
    NCBI Accession
    NP_671723, NP_982288
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