PINK1 antibody (Internal Region)
(1 reference)-
- Target See all PINK1 Antibodies
- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
-
Binding Specificity
- Internal Region
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This PINK1 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- Purpose
- PINK1
- Sequence
- QGKAHLESRS YQEAQ
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QGKAHLESRSYQEAQ, from the internal region of the protein sequence according to NP_115785.1.
- Isotype
- IgG
-
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibody
PINK1 Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A9F8 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) antibodyPINK1 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Mouse Monoclonal 38CT20-8-5 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-273) antibodyPINK1 Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5F2B11 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 118-147), (N-Term) antibodyPINK1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB07381 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibodyPINK1 Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S4-15 unconjugated
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (HRP)PINK1 Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S4-15 HRP
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (FITC)PINK1 Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S4-15 FITC
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Alkaline Phosphatase (AP))PINK1 Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S4-15 Alkaline Phosphatase (AP)
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (Biotin)PINK1 Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S4-15 Biotin
anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 112-496) antibody (PE)PINK1 Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S4-15 PE
-
- Application Notes
-
Western Blot: Approx. 60-65 kDa band observed in lysates of cell lines Jurkat and HeLa (calculated MW of 62.8 kDa according to NP_115785.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
-
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
: "BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria." in: Autophagy, Vol. 10, Issue 6, pp. 1105-19, (2014) (PubMed).
-
: "BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria." in: Autophagy, Vol. 10, Issue 6, pp. 1105-19, (2014) (PubMed).
-
- Target
- PINK1 (PTEN Induced Putative Kinase 1 (PINK1))
- Alternative Name
- PINK1 (PINK1 Products)
- Background
- PINK1, PTEN induced putative kinase 1 , BRPK, FLJ27236, PARK6 , protein kinase BRPK, serine/threonine-protein kinase PINK1
- Gene ID
- 65018, 68943, 298575
- NCBI Accession
- NP_115785
- Pathways
- Autophagy
-
