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TOP2 antibody (C-Term, N-Term)

TOP2 Reactivity: Arabidopsis thaliana, Vicia faba WB, IL Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN334538
  • Target See all TOP2 Antibodies
    TOP2 (DNA Topoisomerase II (TOP2))
    Binding Specificity
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term, N-Term
    Reactivity
    • 13
    • 2
    • 1
    Arabidopsis thaliana, Vicia faba
    Host
    • 11
    • 5
    Rabbit
    Clonality
    • 10
    • 6
    Polyclonal
    Conjugate
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    This TOP2 antibody is un-conjugated
    Application
    • 16
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunolocalization (IL)
    Specificity
    Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.
    Cross-Reactivity (Details)
    Not reactive in: Nicotiana tabacum
    Predicted Reactivity
    Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)
    Purification
    serum
    Immunogen
    The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.
    Top Product
    Discover our top product TOP2 Primary Antibody
  • Application Notes
    1: 2000 (WB), 1: 500 (IL)
    Comment

    Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 μL of sterile water
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

  • Target
    TOP2 (DNA Topoisomerase II (TOP2))
    Alternative Name
    TOP2 (TOP2 Products)
    Synonyms
    Topoii antibody, ptr11 antibody, DDBDRAFT_0191045 antibody, DDBDRAFT_0231510 antibody, DDB_0191045 antibody, DDB_0231510 antibody, CG10223 antibody, CHRAC antibody, Dmel\\CG10223 antibody, TOP2 antibody, TOPO II antibody, TOPOII antibody, TopII antibody, Topo II antibody, TopoII antibody, dCHRAC antibody, dTopoII antibody, top2 antibody, topo II antibody, topoII antibody, topoisomerase II antibody, DNA topoisomerase II antibody, DNA topoisomerase 2 antibody, DNA topoisomerase ii antibody, type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit antibody, Topoisomerase 2 antibody, TOP2 antibody, A583L antibody, top2 antibody, CNA03600 antibody, Tb09.160.4090 antibody, Tb11.01.3390 antibody, LMJF_28_2280 antibody, Top2 antibody
    Background
    AGI Code: At3g23890
    Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II
    Molecular Weight
    expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)
    UniProt
    P30182
    Pathways
    Mitotic G1-G1/S Phases
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