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PLAAT3 (N-Term) antibody

Verified Reactivity: Human ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN335142
  • Target
    PLAAT3
    Binding Specificity
    N-Term
    Reactivity
    Human
    Host
    Goat
    Clonality
    Polyclonal
    Application
    ELISA, Immunohistochemistry (IHC)
    Purpose
    HRASLS3 / HREV107-3
    Sequence
    RAPIPEPKPG DLIE
    Specificity
    Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence RAPIPEPKPGDLIE-C, from the N Terminus of the protein sequence according to NP_009000.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

    Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Heart lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.0k

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    PLAAT3
    Background
    PLA2G16, phospholipase A2, group XVI, AdPLA, H-REV107-1, HRASLS3, HREV107, HREV107-3, MGC118754, HRAS-like suppressor 3, adipose-specific PLA2
    Gene ID
    11145, 225845, 24913
    NCBI Accession
    NP_009000
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