NR1I2 antibody (Internal Region)

Catalog No. ABIN335159

Product Details anti-NR1I2 Antibody

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR1I2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: PXR / NR1I2

Sequence: EQFAITLKSY IECNR

Specificity: This antibody is expected to recognize all reported isoforms (NP_071285.1, NP_148934.1, NP_003880.3).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3, NP_071285.1, NP_148934.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx. 48 kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8 kDa according to NP_003880.3). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 35 kDa. This band wa

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR1I2

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Alternative Name: NR1I2 (NR1I2 Products)

Synonyms: BXR antibody, ONR1 antibody, PAR antibody, PAR1 antibody, PAR2 antibody, PARq antibody, PRR antibody, PXR antibody, SAR antibody, SXR antibody, PXR.1 antibody, PXR.2 antibody, PXR1 antibody, mPXR antibody, bxr antibody, nr1i2 antibody, onr1 antibody, xonr1 antibody, nuclear receptor subfamily 1 group I member 2 antibody, nuclear receptor subfamily 1, group I, member 2 antibody, nuclear receptor subfamily 1 group I member 2 S homeolog antibody, NR1I2 antibody, Nr1i2 antibody, nr1i2.S antibody, nr1i2 antibody

Background: PXR, NR1I2, nuclear receptor subfamily 1, group I, member 2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, SAR, SXR, orphan nuclear receptor, pregnane X receptor, steroid and xenobiotic receptor

Gene ID: 8856

NCBI Accession: NP_003880, NP_071285, NP_148934

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway