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Goat anti-Rat IgG Antibody (Alkaline Phosphatase (AP))

ELISA, IHC, WB, ICC, IA Polyclonal Alkaline Phosphatase (AP)
Catalog No. ABIN336770
  • Target See all IgG products
    IgG
    Reactivity
    • 692
    • 672
    • 559
    • 451
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 1717
    • 1474
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Conjugate
    • 556
    • 408
    • 406
    • 367
    • 297
    • 276
    • 228
    • 120
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 31
    • 31
    • 30
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Alkaline Phosphatase (AP)
    Application
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 514
    • 233
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)
    Purification
    Affinity purified using solid phase Rat IgG
    May contain small amounts of intact IgG
    Purity
    > 90 % based on SDS-PAGE
    Immunogen
    Goat serum was obtained from animals of US origin and under the care of a registered veterinarian.
  • Application Notes
    This conjugate is suitable for all immunoassay applications.
    The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry
    Comment

    ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

    Restrictions
    For Research Use only
  • Buffer
    30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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