GNAI1 antibody
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- Target See all GNAI1 Antibodies
- GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))
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Reactivity
- Human, Mouse, Rat, Cow, Guinea Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GNAI1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.
- Purification
- Protein A purified
- Immunogen
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Partially purified Gai1 protein from rat brain (39-42kD). Cellular Localization: Cell membrane.
Type of Immunogen: Protein fraction - Clone
- R4-5
- Isotype
- IgG2b
- Top Product
- Discover our top product GNAI1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (1:50), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Positive control: IMR-5 cells, brain. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.2 % BSA, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))
- Alternative Name
- GNAI1 / Gi (GNAI1 Products)
- Synonyms
- AU046200 antibody, Gialpha1 antibody, Gnai-1 antibody, gnai1 antibody, Gi antibody, BPGTPB antibody, zgc:63957 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting 1 antibody, G protein subunit alpha i1 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 S homeolog antibody, guanine nucleotide-binding protein G(i) subunit alpha-1 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 antibody, Gnai1 antibody, gnai1.S antibody, GNAI1 antibody, LOC397505 antibody, gnai1 antibody
- Background
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Name/Gene ID: GNAI1
Synonyms: GNAI1, Gi1 protein alpha subunit, Gi - Gene ID
- 2770
- UniProt
- P63096
- Pathways
- G-protein mediated Events
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